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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
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JoVE Journal 生化学
Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

DOI:
10.3791/55762-v

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09:13 min

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June 14, 2017

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, , , , , ,
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan,Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section,University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit,Centro Cardiologico Monzino IRCCS

章

  • 00:05標題
  • 01:17Mitral Valve Preparation
  • 03:39Protein Extraction
  • 07:20Results: Four Newly Identified Mitral Valve Proteins
  • 08:28Conclusion

概要

自動翻訳

自動翻訳

The protein composition of the human mitral valve is still partially unknown, because its analysis is complicated by low cellularity and therefore by low protein biosynthesis. This work provides a protocol to efficiently extract protein for the analysis of the mitral valve proteome.

タグ

Protein ExtractionMitral ValveCardiac Valve DiseaseProteomic AnalysisDiagnostic MarkersTherapeutic TargetsPorcine Mitral ValveExplanted HeartLeft AtriumLeft VentricleAnterior Mitral Valve LeafletPosterior Mitral Valve LeafletCommissuresDissectionProtein Extraction Workflow

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