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Assaying Protein Kinase Activity with Radiolabeled ATP
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May 26, 2017
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概要
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Protein kinases are highly evolved signaling enzymes and scaffolds that are critical for inter- and intracellular signal transduction. We present a protocol for measuring kinase activity through the use of radiolabeled adenosine triphosphate ([γ-32P] ATP), a reliable method to aid in elucidation of cellular signaling regulation.
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Karra, A. S., Stippec, S., Cobb, M. H. Assaying Protein Kinase Activity with Radiolabeled ATP. J. Vis. Exp. (123), e55504, doi:10.3791/55504 (2017).
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