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Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
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JoVE Journal 生物学
Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

DOI:
10.3791/55471-v

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07:38 min

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April 09, 2017

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, ,
1MRC Laboratory for Molecular Cell Biology,University College London, 2Centre for Mathematics and Physics in Life Sciences and Experimental Biology (CoMPLEX),University College London, 3Department of Cell and Developmental Biology,University College London

章

  • 00:05標題
  • 00:50Image Setup and Particle Extraction
  • 02:53Seed Generation and Selection
  • 04:32Model Generation from Seeds
  • 06:11Results: Modeling of Vaccinia with VirusMapper
  • 06:49Conclusion

概要

自動翻訳

自動翻訳

This manuscript uses the Fiji-based open-source software package VirusMapper to apply single-particle analysis to super-resolution microscopy images in order to generate precise models of nanoscale structure.

タグ

Single-particle AnalysisSuper-resolution MicroscopyVirusMapperVirus StructureMacromolecular ComplexesFluorescence MicroscopyImage ProcessingParticle ExtractionROI SelectionSeed Generation

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