The American bullfrog's (Rana catesbeiana) sacculus permits direct examination of hair-cell physiology. Here the dissection and preparation of the bullfrog's sacculus for biophysical studies is described. We show representative experiments from these hair cells, including the calculation of a bundle's force-displacement relation and measurement of its unforced motion.
Azimzadeh, J. B., Salvi, J. D. Physiological Preparation of Hair Cells from the Sacculus of the American Bullfrog (Rana catesbeiana). J. Vis. Exp. (121), e55380, doi:10.3791/55380 (2017).