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Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems
DOI:

09:58 min

December 08, 2017

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  • 00:05標題
  • 00:45Exposure of Pre-filtration Sentinels Out of a Recirculating System
  • 02:41Sump Swabs
  • 03:42P. tomentosa Eggs Detection at the Bottom of a Tank and Animal Screening
  • 06:48PCR on Sludge Sediment
  • 08:12Results: Sump Swabs are Valuable Alternative to Identify Prevalent Mycobacterium
  • 09:21Conclusion

概要

自動翻訳

This protocol describes the use of sump swabs and sludge analysis of zebrafish systems, which leads to increased detection compared to the sole use of sentinels to detect pathogens such as Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa. A system to monitor P. tomentosa eggs in quarantine is also proposed.

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