ジャーナル
/
神経科学
/
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

DOI:
10.3791/55176-v

•

11:36 min

•

February 10, 2017

•

, , , , ,
1Department of Neuroscience of Disease, Center for Transdisciplinary Research,Niigata University, 2Brain Research Institute,Niigata University, 3Image and Data Analysis Facility,German Center for Neurodegenerative Diseases (DZNE), 4Graduate School of Life Science and Technology,Tokyo Institute of Technology (Titech), 5Dendrite Differentiation,German Center for Neurodegenerative Diseases (DZNE)

章

  • 00:05標題
  • 00:47Treatment of Flies and Imaging Brains
  • 02:54Image Analysis: Generating the Spots, Surface Objects, Start-points, and End-points
  • 08:04Image Analysis: Calculating the Number of Spots, Distribution of Spot Frequency and Enrichment Level of Brp-GFP
  • 09:48Results: Brp-GFP Expression in R8 Synapses of the Medulla
  • 11:02Conclusion

概要

自動翻訳

自動翻訳

Here we show how to quantify the number and spatial distribution of synaptic active zones in Drosophila melanogaster photoreceptors, highlighted with a genetically encoded molecular marker, and their modulation after prolonged exposure to light.

タグ

Synaptic ModulationDrosophila MelanogasterPhotoreceptorsProlonged Light ExposureSynaptic PlasticitySynaptic DynamicsNeuron ActivitySynaptic AnalysisBrp ExpressionR7 And R8 Photoreceptor AxonsImmunolabeling3D ImagingImage AnalysisSpot Detection

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

Conditions Affecting Social Space in Drosophila melanogaster

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

Desensitization and Recovery of Crayfish Photoreceptors Upon Delivery of a Light Stimulus

Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

Read Article