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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
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JoVE Journal 遺伝学
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

8,326 Views

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08:06 min

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January 19, 2017

DOI:

10.3791/55157-v

DOI:
10.3791/55157-v

•

08:06 min
January 19, 2017

•

3 Views
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1Dept. of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine,Hebrew University of Jerusalem, 2The Core Research Facility, IMRIC, Faculty of Medicine,Hebrew University of Jerusalem

概要

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We describe here a relatively fast and simple approach for mapping genome-wide mammalian replication timing, from cell isolation to the basic analysis of the sequencing results. A genomic map of a representative replication program will be provided following the protocol.

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Yehuda, Y., Blumenfeld, B., Lehmann, D., Simon, I. Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement. J. Vis. Exp. (119), e55157, doi:10.3791/55157 (2017).

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