ジャーナル
/
免疫学と感染
/
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

10,893 Views

•

08:34 min

•

February 22, 2017

DOI:

10.3791/55146-v

DOI:
10.3791/55146-v

•

08:34 min
February 22, 2017

•

9 Views
, , , , ,
1Institute for Lung Research, Universities of Giessen and Marburg Lung Center,Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine,University Medical Center Giessen and Marburg

概要

Automatically generated

Here, we describe the purification of Legionella pneumophila (L. pneumophila) outer membrane vesicles (OMVs) from liquid cultures. These purified vesicles are then used for the treatment of macrophages to analyze their pro-inflammatory potential.

Explore More Videos

Legionella PneumophilaOuter Membrane Vesicles (OMVs)MacrophagesPro-inflammatory PotentialIsolationPurificationStandardized ProtocolHost-pathogen Interaction

Read Article

Cite this Article

Jung, A. L., Hoffmann, K., Herkt, C. E., Schulz, C., Bertrams, W., Schmeck, B. Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages. J. Vis. Exp. (120), e55146, doi:10.3791/55146 (2017).

Download .ris file

Copy

Share Video

.

Copy