Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Jonathan Fischer; Roger A. Johnson; Elizabeth Boon

doi:10.3791/55129

ジャーナル

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生化学

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Quantificazione dei batteri istidina chinasi autophosphorylation usando un test nitrocellulosa Binding

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生化学

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JoVE Journal 生化学

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

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Quantificazione dei batteri istidina chinasi autophosphorylation usando un test nitrocellulosa Binding

8,406 Views

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09:17 min

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January 11, 2017

DOI:

10.3791/55129-v

DOI:

10.3791/55129-v

•

09:17 min

January 11, 2017

•

1 Views

Jonathan Fischer¹, Roger A. Johnson², Elizabeth Boon¹

¹化学科,Stony Brook University, ²Department of Physiology and Biophysics,Stony Brook University

概要

We report and demonstrate an optimized nitrocellulose binding assay that can be used to quantify autophosphorylation of purified bacterial histidine kinases. Our method has several advantages over traditional SDS-PAGE based techniques, providing a valuable alternative for characterizing these important proteins.

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Quantificazione dei batteri istidina chinasi autophosphorylation usando un test nitrocellulosa Binding

概要

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