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使用中性毛细管单克隆抗体亚型的毛细管电泳分离
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生化学
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JoVE Journal 生化学
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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使用中性毛细管单克隆抗体亚型的毛细管电泳分离

12,456 Views

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14:53 min

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January 16, 2017

DOI:

10.3791/55082-v

DOI:
10.3791/55082-v

•

14:53 min
January 16, 2017

•

3 Views
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1Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.

概要

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Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.

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Capillary ElectrophoresisMonoclonal AntibodyIsoformsHPMCBuffer SolutionSample PreparationCE Instrument

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Cite this Article

Espinosa-de la Garza, C. E., Salazar-Flores, R. D., Pérez, N. O., Flores-Ortiz, L. F., Medina-Rivero, E. Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary. J. Vis. Exp. (119), e55082, doi:10.3791/55082 (2017).

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