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İşitsel Ayrımcılık Öğrenme sonra Fare Beyin Bölgeler Yüksek Çözünürlüklü Sayısal Synaptic Proteome Profil
JoVE Journal
神経科学
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JoVE Journal 神経科学
High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

İşitsel Ayrımcılık Öğrenme sonra Fare Beyin Bölgeler Yüksek Çözünürlüklü Sayısal Synaptic Proteome Profil

DOI:

10:36 min

December 15, 2016

, , , , , , , ,

  • 00:05標題
  • 01:14Auditory Discrimination Learning in the Shuttle Box (FMTD Paradigm)
  • 02:28Brain Dissection
  • 03:43Purification of Synaptosomes from Crude Brain Membrane Fractions
  • 06:29Sample Preparation for Mass Spectrometry Using In-solution Digest
  • 09:02Results: Synaptic Abundance of selected Proteins After FMTD Task Training
  • 09:53Conclusion

概要

自動翻訳

The identification of molecules and pathways controlling synaptic plasticity and memory is still a major challenge in neuroscience. Here, a workflow is described addressing the relative quantification of synaptic proteins supposedly involved in the molecular reorganization of synapses during learning and memory consolidation in an auditory learning paradigm.

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