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タンパク質機能を調べるための誘導LAP-タグ付けされた安定な細胞株、時空間局在とタンパク質相互作用ネットワーク
JoVE Journal
生物学
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JoVE Journal 生物学
Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
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タンパク質機能を調べるための誘導LAP-タグ付けされた安定な細胞株、時空間局在とタンパク質相互作用ネットワーク

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11:04 min

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December 24, 2016

DOI:

10.3791/54870-v

DOI:
10.3791/54870-v

•

11:04 min
December 24, 2016

•

2 Views
, , , ,
1Department of Chemistry and Biochemistry,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles, 3Jonsson Comprehensive Cancer Center,University of California, Los Angeles

概要

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We describe a method for generating localization and affinity purification (LAP)-tagged inducible stable cell lines for investigating protein function, spatiotemporal subcellular localization and protein-protein interaction networks.

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Inducible LAP-taggedStable Cell LinesProtein FunctionSpatiotemporal LocalizationProtein Interaction NetworksHEK293 CellsHygromycin SelectionTandem Affinity Purification (TAP)Tet/Dox InductionAnti-GFP AntibodyProtein A Beads

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Bradley, M., Ramirez, I., Cheung, K., Gholkar, A. A., Torres, J. Z. Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks. J. Vis. Exp. (118), e54870, doi:10.3791/54870 (2016).

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