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Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
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JoVE Journal 免疫学と感染
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

12,975 Views

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09:35 min

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February 01, 2017

DOI:

10.3791/54849-v

DOI:
10.3791/54849-v

•

09:35 min
February 01, 2017

•

12 Views
, , , , ,
1Research Center for Hepatitis and Immunology,National Center for Global Health and Medicine, 2Central Pharmaceutical Research Institute,Japan Tobacco Inc.

概要

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Here we describe a newly developed hepatitis B virus (HBV) reporter system to monitor the early stages of the HBV life cycle. This simplified in vitro system will aid in the screening of anti-HBV agents using a high-throughput strategy.

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Hepatitis B VirusHBVReporter SystemHepG2 CellsTransfectionRecombinant HBVCell CultureVirus ReplicationVirus PurificationNTCPInfection

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Cite this Article

Nishitsuji, H., Yamamoto, H., Shiina, R., Harada, K., Ujino, S., Shimotohno, K. Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle. J. Vis. Exp. (120), e54849, doi:10.3791/54849 (2017).

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