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Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
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JoVE Journal 免疫学と感染
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

DOI:
10.3791/54849-v

•

09:35 min

•

February 01, 2017

•

, , , , ,
1Research Center for Hepatitis and Immunology,National Center for Global Health and Medicine, 2Central Pharmaceutical Research Institute,Japan Tobacco Inc.

章

  • 00:05標題
  • 00:42Production of Recombinant Hepatitis B Virus Encoding the Reporter Protein
  • 03:33Infection of Recombinant Hepatitis B Virus
  • 05:09Analysis of Hepatitis B Virus Infection
  • 05:56Results: HBV Reporter Activity in HepG2 Cells Infected with Recombinant Hepatitis B Virus
  • 07:57Conclusion

概要

自動翻訳

自動翻訳

Here we describe a newly developed hepatitis B virus (HBV) reporter system to monitor the early stages of the HBV life cycle. This simplified in vitro system will aid in the screening of anti-HBV agents using a high-throughput strategy.

タグ

Hepatitis B VirusHBVReporter SystemHepG2 CellsTransfectionRecombinant HBVCell CultureVirus ReplicationVirus PurificationNTCPInfection

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