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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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January 02, 2017
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An optimized protocol is presented for the generation of monoclonal antibodies based on the hybridoma technology. Mice were immunized with an immunoconjugate. Spleen cells were fused by PEG and an electric impulse with immortal myeloma cells. Antibody-producing hybridoma cells were selected by HAT and antigen-specific ELISA screening.
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Holzlöhner, P., Hanack, K. Generation of Murine Monoclonal Antibodies by Hybridoma Technology. J. Vis. Exp. (119), e54832, doi:10.3791/54832 (2017).
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