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Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
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生物学
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JoVE Journal 生物学
Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

DOI:
10.3791/54717-v

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10:18 min

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November 01, 2016

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,
1Department of Plant Sciences,University of Oxford

章

  • 00:05標題
  • 00:46Growth of Arabidopsis Plants and the Stress Treatment
  • 01:34Chloroplast Isolation
  • 05:06Analysis of the Yield and the Intactness of Chloroplasts
  • 06:19Chloroplast Protein Import
  • 08:04Results: Chloroplast Import of the PsaD Protein Is Elevated in the sp1 Mutant under Stress Conditions
  • 09:12Conclusion

概要

自動翻訳

自動翻訳

Here we describe a new method to study protein import into isolated chloroplasts under stress. The method is rapid and straightforward, and can be applied to study the consequences of different stress conditions for chloroplast protein import, and the corresponding regulatory mechanisms.

タグ

Protein ImportChloroplastsStressed PlantsArabidopsis ThalianaProtein Import RegulationChloroplast IsolationPercoll GradientProtein Import Assay

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