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Inducing Complete Polyp Regeneration from the Aboral Physa of the Starlet Sea Anemone Nematostella vectensis
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Inducing Complete Polyp Regeneration from the Aboral Physa of the Starlet Sea Anemone Nematostella vectensis

Inducing Complete Polyp Regeneration from the Aboral Physa of the Starlet Sea Anemone Nematostella vectensis

DOI:

08:17 min

January 14, 2017

,

  • 00:05標題
  • 01:05Selection and Relaxation of Nutritionally Conditioned Animals
  • 02:12Amputation
  • 03:44Assessing Regeneration with the Nematostella Regeneration Staging System (NRSS)
  • 05:47Results: Effects of Chloroquine, and Inhibitor of Autophagy
  • 06:35Conclusion

概要

自動翻訳

Here we demonstrate how to induce and monitor regeneration in the Starlet Sea Anemone Nematostella vectensis, a model cnidarian anthozoan. We demonstrate how to amputate and categorize regeneration using a morphological staging system, and we use this system to reveal a requirement for autophagy in regenerating polyp structures.

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