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In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments
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10:32 min
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October 25, 2016
DOI:
1Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology,University of Antwerp, 2Visceral Pain Group, Discipline of Medicine,University of Adelaide, 3生物医科学科,University of Sheffield, 4Department of Pharmacy, Pharmacology and Postgraduate Medicine,University of Hertfordshire, 5Department of Gastroenterology and Hepatology,Antwerp University Hospital
概要
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Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.
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Nullens, S., Deiteren, A., Jiang, W., Keating, C., Ceuleers, H., Francque, S., Grundy, D., De Man, J. G., De Winter, B. Y. In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments. J. Vis. Exp. (116), e54576, doi:10.3791/54576 (2016).
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