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Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition
章
- 00:05標題
- 00:45Preparing Agar Plates, Sterile Broth, and Sterile Vapoizer Bottles
- 02:19Prepare Bacterial Overnight Cultures and Spot Culture of the Inhibitor-producing Isolate
- 03:34Preparing Spray Inoculum of Test Competitor Strains and Deferred Growth Inhibition Assay
- 04:48Results: Antimicrobial-derived Deferred Growth Inhibition
- 06:16Conclusion
The deferred growth inhibition assay can be used to assess the competition effect by one bacterial isolate on another. Inhibition is quantified by measuring the zone of clearing around the inhibitor-producing isolate, or qualitatively assessed by determining the visible extent of inhibition.
