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Measuring Lactase Enzymatic Activity in the Teaching Lab
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JoVE Journal 生物学
Measuring Lactase Enzymatic Activity in the Teaching Lab

Measuring Lactase Enzymatic Activity in the Teaching Lab

109,531 Views

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04:41 min

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August 06, 2018

DOI:

10.3791/54377-v

DOI:
10.3791/54377-v

•

04:41 min
August 06, 2018

•

192 Views
, , , , ,
1School of Pharmacy,University of Reading, 2Department of Molecular Neuroscience,UCL Institute of Neurology, 3School of Biological Sciences, Royal Hollway,University of London

概要

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The enzymatic activity of lactase is essential for the catabolic processing of the disaccharide lactose. Here, the activity of lactase found in dietary supplements is assayed using a colorimetric assay. This provides students with an experimental platform for understanding the activity of lactase and enzyme kinetics.

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Cite this Article

Leksmono, C. S., Manzoni, C., Tomkins, J. E., Lucchesi, W., Cottrell, G., Lewis, P. A. Measuring Lactase Enzymatic Activity in the Teaching Lab. J. Vis. Exp. (138), e54377, doi:10.3791/54377 (2018).

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