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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis

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09:38 min

October 16, 2016

DOI:

09:38 min
October 16, 2016

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概要

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We present transient transgenesis and gene knockdown in Ciona intestinalis, a chordate sister group to vertebrates, using microinjection and electroporation techniques. Such methods facilitate functional genomics in this simple invertebrate that features rudimentary characteristics of vertebrates, including notochord and head sensory epithelia, and many orthologs of human disease associated genes.

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