ジャーナル
/
免疫学と感染
/
に関与する宿主因子のハイスループットスクリーニングのための顕微鏡ベースのアッセイ<em>ブルセラ</em> HeLa細胞の感染
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Please note that all translations are automatically generated. Click here for the English version.

に関与する宿主因子のハイスループットスクリーニングのための顕微鏡ベースのアッセイ<em>ブルセラ</em> HeLa細胞の感染

8,138 Views

•

15:29 min

•

August 05, 2016

DOI:

10.3791/54263-v

DOI:
10.3791/54263-v

•

15:29 min
August 05, 2016

•

1 Views
, , , , , ,
1Focal Area Infection Biology, Biozentrum,University of Basel, 2Centre d’Immunologie de Marseille-Luminy,Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical,Universidad de Navarra, 4BioDataAnalysis GmbH

概要

Automatically generated

Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.

Explore More Videos

MicroscopyHigh-throughput ScreeningBrucella InfectionHeLa CellsHost FactorsSiRNACell CultureTransfectionAutomated WorkflowImage Analysis

Read Article

Cite this Article

Casanova, A., Low, S. H., Emmenlauer, M., Conde-Alvarez, R., Salcedo, S. P., Gorvel, J., Dehio, C. Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells. J. Vis. Exp. (114), e54263, doi:10.3791/54263 (2016).

Download .ris file

Copy

Share Video

.

Copy