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Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System
章
- 00:05標題
- 00:36Preparation of Single-cohort Colonies
- 02:28Classifying Hypopharyngeal Glands (HPGs)
- 04:09Injection of Active Ecdysone (20E)
- 05:24Application of Methoprene, a JH Analogue
- 06:23Results: mrjp2 and Hbg3 Expression in the HPG of Worker Honeybees
- 07:48Conclusion
Here we describe our detailed protocol for the preparation of single-cohort honeybee colonies – a useful tool for analyzing the role-associated worker physiology. We also describe detailed protocols for treating workers with juvenile hormone and ecdysone to evaluate the involvement of these hormones in the regulation of worker behavior and/or physiology.
