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셀룰로오스 종이 디스크와 벌거 벗은 눈 색채 면역 측정법에 그들의 응용 프로그램에 항체의 결합 공유
JoVE Journal
生化学
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JoVE Journal 生化学
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

셀룰로오스 종이 디스크와 벌거 벗은 눈 색채 면역 측정법에 그들의 응용 프로그램에 항체의 결합 공유

DOI:

09:04 min

October 21, 2016

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  • 00:05標題
  • 00:39Grafting Amine Functional Groups on Cellulose Paper Discs
  • 03:10Paper-based ELISA for IgG Detection
  • 06:28Results: Analysis of the Binding Activity of Immobilized Antibodies to the Covalent Immobilized Cellulose Paper Matrix
  • 08:21Conclusion

概要

自動翻訳

In this manuscript, periodate oxidation and glutaraldehyde cross-linking methods for covalent immobilization of antibodies on paper discs are presented. The binding activity of immobilized antibodies was evaluated. Based on these results, a glutaraldehyde cross-linking method was used to develop a novel paper-based immunoassay for immunoglobulin G (IgG) detection.

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