This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.
Liu, C., Chan, C. An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons. J. Vis. Exp. (114), e54085, doi:10.3791/54085 (2016).