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The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
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JoVE Journal 免疫学と感染
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

13,325 Views

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09:09 min

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May 18, 2016

DOI:

10.3791/54033-v

DOI:
10.3791/54033-v

•

09:09 min
May 18, 2016

•

3 Views
, , , ,
1Department of Biomedical Sciences and Pathobiology,Virginia Maryland College of Veterinary Medicine, Virginia Tech, 2Department of Biomedical Engineering,Cornell University, 3School of Electrical and Computer Engineering,Cornell University, 4Department of Biomedical Engineering,Duke University

概要

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Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.

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Cite this Article

Rothschild, D. E., Srinivasan, T., Aponte-Santiago, L. A., Shen, X., Allen, I. C. The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids. J. Vis. Exp. (111), e54033, doi:10.3791/54033 (2016).

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