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Previsão e Validação de elementos reguladores de genes ativada durante ácido retinóico induzida estaminais embrionárias Diferenciação Celular
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JoVE Journal 発生生物学
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Previsão e Validação de elementos reguladores de genes ativada durante ácido retinóico induzida estaminais embrionárias Diferenciação Celular

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09:07 min

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June 21, 2016

DOI:

10.3791/53978-v

DOI:
10.3791/53978-v

•

09:07 min
June 21, 2016

•

4 Views
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1Sanford-Burnham-Prebys Medical Discovery Institute at Lake Nona, 2生化学・分子生物学科,Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, 3MTA-DE “Lendulet” Immunogenomics Research Group,University of Debrecen

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In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.

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Gene Regulatory ElementsEnhancersRetinoic AcidEmbryonic Stem Cell DifferentiationReporter Plasmid ConstructTransfectionTranscription Regulation

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Cite this Article

Simandi, Z., Horvath, A., Nagy, P., Nagy, L. Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation. J. Vis. Exp. (112), e53978, doi:10.3791/53978 (2016).

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