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Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

8,088 Views

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09:07 min

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June 21, 2016

DOI:

10.3791/53978-v

DOI:
10.3791/53978-v

•

09:07 min
June 21, 2016

•

4 Views
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1Sanford-Burnham-Prebys Medical Discovery Institute at Lake Nona, 2Department of Biochemistry and Molecular Biology,Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, 3MTA-DE “Lendulet” Immunogenomics Research Group,University of Debrecen

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In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.

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Gene Regulatory ElementsEnhancersRetinoic AcidEmbryonic Stem Cell DifferentiationReporter Plasmid ConstructTransfectionTranscription Regulation

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Simandi, Z., Horvath, A., Nagy, P., Nagy, L. Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation. J. Vis. Exp. (112), e53978, doi:10.3791/53978 (2016).

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