ジャーナル
/
化学
/
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
JoVE Journal
化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 化学
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

DOI:
10.3791/53747-v

•

08:42 min

•

February 19, 2016

•

, ,
1生物学科,Virginia Commonwealth University, 2Center for Human Disease Modeling,Duke University Medical Center

章

  • 00:05標題
  • 00:33Embryo Fixation
  • 01:26Immunostaining Whole Embryos
  • 03:47Confocal Imaging and Processing
  • 06:37Results: Immunofluorescence of Phospho-epitopes in Whole Mount Zebrafish Ciliated Organs
  • 08:11Conclusion

概要

自動翻訳

自動翻訳

Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.

タグ

ImmunostainingPhospho-epitopesCiliated OrgansWhole MountZebrafish EmbryosCalcium-dependent SignalingImmunolocalizationPhosphorylated ProteinsAcetylated TubulinAnti-phospho CaMKII AntibodyFluorescent Secondary Antibody発生生物学Cell Biology

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

High Precision Zinc Isotopic Measurements Applied to Mouse Organs

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Accessing Valuable Ligand Supports for Transition Metals: A Modified, Intermediate Scale Preparation of 1,2,3,4,5-Pentamethylcyclopentadiene

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Comparison of Two Different Synthesis Methods of Single Crystals of Superconducting Uranium Ditelluride

Read Article