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Identificación de las nanopartículas de óxido de metal en muestras histológicas por Enhanced Mapeo de campo oscuro Microscopía y hiperespectrales
JoVE Journal
生物工学
This content is Free Access.
JoVE Journal 生物工学
Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

Identificación de las nanopartículas de óxido de metal en muestras histológicas por Enhanced Mapeo de campo oscuro Microscopía y hiperespectrales

DOI:

12:19 min

December 08, 2015

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  • 00:05標題
  • 02:03Initializing the Microscope
  • 03:05Capturing Images
  • 04:13Capturing Datacubes
  • 06:07Creation of Reference Spectral Libraries
  • 07:59Image Analysis: Spectral Angle Mapping
  • 10:04Results: Hyperspectral Microscope Positively Identifies Nanoparticles
  • 11:25Conclusion

概要

自動翻訳

Enhanced darkfield microscopy and hyperspectral imaging with spectral mapping enable screening, localization, and identification of nanoscale materials in histological samples with improved speed and accuracy over traditional methods. The goal of this paper is to provide methods for darkfield imaging and hyperspectral mapping of metal oxide nanoparticles in histological samples.

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