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Précis et phénol libre ADN sexage des embryons de porcins Jour 30 par PCR
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Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
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Précis et phénol libre ADN sexage des embryons de porcins Jour 30 par PCR

9,852 Views

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10:16 min

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February 14, 2016

DOI:

10.3791/53301-v

DOI:
10.3791/53301-v

•

10:16 min
February 14, 2016

•

6 Views
, ,
1Department of Agricultural, Food and Nutritional Science,University of Alberta

概要

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This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.

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DNA SexingPCRPorcine EmbryosPhenol-freeReproductive PhysiologySex RatioDNA PurificationEmbryo SamplesLiquid NitrogenSodium HydroxideDNA Lysate

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Cite this Article

Blanes, M. S., Tsoi, S. C., Dyck, M. K. Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR. J. Vis. Exp. (108), e53301, doi:10.3791/53301 (2016).

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