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Met behulp Isolated Mitochondriën van geringe hoeveelheden van de muis skeletspieren voor High throughput Microplate Respiratory Metingen
JoVE Journal
発生生物学
This content is Free Access.
JoVE Journal 発生生物学
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

Met behulp Isolated Mitochondriën van geringe hoeveelheden van de muis skeletspieren voor High throughput Microplate Respiratory Metingen

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10:39 min

October 30, 2015

DOI:

10:39 min
October 30, 2015

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概要

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The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

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