このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
La purificación de los buques de cerebro de ratón
22,717 Views
•
07:32 min
•
November 10, 2015
DOI:
1Collège de France, Center for Interdisciplinary Research in Biology (CIRB), Centre National de la Recherche Scientifique CNRS, Unité Mixte de Recherche 7241,Institut National de la Santé et de la Recherche Médicale, 2Centre Interdisciplinaire de Recherche en Biologie, 3MEMOLIFE Laboratory of Excellence and Paris Science, Lettre Research University, 4Université Paris Descartes, Faculté de Pharmacie,Université Paris Diderot
概要
Automatically generated
We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.
Read Article
Cite this Article
Boulay, A., Saubaméa, B., Declèves, X., Cohen-Salmon, M. Purification of Mouse Brain Vessels. J. Vis. Exp. (105), e53208, doi:10.3791/53208 (2015).
Copy