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إعداد الميتوكوندريا المخصب الكسور لتحليل التمثيل الغذائي في<em> ذبابة الفاكهة</em
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JoVE Journal 発生生物学
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
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إعداد الميتوكوندريا المخصب الكسور لتحليل التمثيل الغذائي في<em> ذبابة الفاكهة</em

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08:22 min

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September 30, 2015

DOI:

10.3791/53149-v

DOI:
10.3791/53149-v

•

08:22 min
September 30, 2015

•

3 Views
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1Biology Department,Adelphi University, 2Department of Ecology and Evolutionary Biology,Brown University

概要

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Mitochondria play central roles in the regulation of metabolism and homeostasis. Subtle changes in mitochondrial metabolism that affect organismal physiology could be difficult to detect in whole organism metabolomics studies. Here we describe an isolation method that enhances the detection of subtle metabolic shifts in Drosophila melanogaster.

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Cite this Article

Villa-Cuesta, E., Rand, D. M. Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila. J. Vis. Exp. (103), e53149, doi:10.3791/53149 (2015).

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