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Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
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生物工学
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JoVE Journal 生物工学
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

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09:38 min

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December 01, 2015

DOI:

10.3791/53073-v

DOI:
10.3791/53073-v

•

09:38 min
December 01, 2015

•

14 Views
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1School of Materials Science and Engineering,Nanyang Technological University, 2Centre for Biomimetic Sensor Science,Nanyang Technological University, 3School of Chemical and Biomedical Engineering,Nanyang Technological University

概要

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We present an experimental protocol to form a supported lipid bilayer on solid substrates without using lipid vesicles. We demonstrate a one-step method to form a lipid bilayer on silicon dioxide and gold as well as supported membranes with cholesterol-enriched domain for various biological applications.

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Biomembrane FabricationSolvent-assisted Lipid BilayerSALB MethodSupported Lipid BilayerSLBVesicle FusionLipid DepositionOrganic SolventHydrophilic SurfaceCholesterol-rich Membranes

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Cite this Article

Tabaei, S. R., Jackman, J. A., Kim, M., Yorulmaz, S., Vafaei, S., Cho, N. Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method. J. Vis. Exp. (106), e53073, doi:10.3791/53073 (2015).

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