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Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall
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July 31, 2015
DOI:
1Division of Drug Delivery and Tissue Engineering,University of Nottingham, 2Laboratory of Biophysics and Surface Analysis, School of Pharmacy,University of Nottingham, 3Division of Immunology and Allergy, School of Molecular Medical Sciences,University of Nottingham, 4Division of Respiratory Medicine, School of Clinical Sciences,University of Nottingham, 5NIHR Respiratory Biomedical Research Unit,University of Leicester, 6School of Sport, Exercise, and Health Sciences,Loughborough University
概要
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Advancements in biomaterial technologies enable the development of three-dimensional multi-cell-type constructs. We have developed electrospinning protocols to produce three individual scaffolds to culture the main structural cells of the airway to provide a 3D in vitro model of the airway bronchiole wall.
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Bridge, J. C., Aylott, J. W., Brightling, C. E., Ghaemmaghami, A. M., Knox, A. J., Lewis, M. P., Rose, F. R., Morris, G. E. Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall. J. Vis. Exp. (101), e52986, doi:10.3791/52986 (2015).
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