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Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
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December 09, 2015
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Drug resistance to targeted therapeutics is widespread and the need to identify mechanisms of resistance--prior to or following clinical onset--is critical for guiding alternative clinical management strategies. Here, we present a protocol to couple derivation of drug-resistant lines in vitro with sequencing to expedite discovery of these mechanisms.
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Korpal, M., Feala, J., Puyang, X., Zou, J., Ramos, A. H., Wu, J., Baumeister, T., Yu, L., Warmuth, M., Zhu, P. Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms. J. Vis. Exp. (106), e52879, doi:10.3791/52879 (2015).
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