ジャーナル
/
免疫学と感染
/
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

DOI:
10.3791/52739-v

•

07:12 min

•

April 16, 2015

•

,
1Department of Microbiology and Immunology, The Chicago Medical School,Rosalind Franklin University of Medicine and Science

章

  • 00:05標題
  • 01:09Mouse Lymph Node and Spleen Isolation
  • 02:11Tissue Processing and CD4+ Enrichment
  • 04:22Naïve CD4+ T Cell Sorting and In vitro Differentiation
  • 05:49Results: Representative In vitro Th Differentiation Data
  • 06:40Conclusion

概要

自動翻訳

自動翻訳

Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.

タグ

MouseNaive CD4+ T CellsT Cell Receptor (TCR)MHC Class IICytokineT Helper Cell DifferentiationT Cell SubsetsIn Vitro DifferentiationEffector T Cells

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Read Article