ジャーナル
/
化学
/
HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
JoVE Journal
化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 化学
HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

DOI:
10.3791/52697-v

•

14:12 min

•

August 01, 2015

•

, , , , , ,
1Environmental Health Science and Research Bureau,Health Canada

章

  • 00:05標題
  • 02:28DNA Extraction, Precipitation and Wash
  • 06:06Enzymatic Digestion
  • 07:44Preparation of Standards, HPLC Run, and Quantification
  • 11:19Results: Optimization of 8-oxo-dG Detection and Quantitation
  • 13:10Conclusion

概要

自動翻訳

自動翻訳

The goal of this protocol is the detection of the DNA oxidation marker, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) by HPLC-ED, in DNA from cultured cells or animal tissues.

タグ

8-oxo-dGuo8-OH-dGuo8-oxo-78-dihydro-2′-deoxyguanosineDNA OxidationOxidative StressHPLC-EDA549 CellsKBrO3Dibenzo(defp)chryseneDBC

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity

Folding and Characterization of a Bio-responsive Robot from DNA Origami

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Determining the Chemical Composition of Corrosion Inhibitor/Metal Interfaces with XPS: Minimizing Post Immersion Oxidation

Fabrication and Testing of Photonic Thermometers

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Disentangling High Strength Copolymer Aramid Fibers to Enable the Determination of Their Mechanical Properties

Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941

Read Article