このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Préparation de Suspensions de cellules uniques pour l'analyse cytofluorimétrie de différentes régions de la peau de souris
17,585 Views
•
05:58 min
•
April 20, 2016
DOI:
概要
Automatically generated
The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.
Read Article
Cite this Article
Broggi, A., Cigni, C., Zanoni, I., Granucci, F. Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions. J. Vis. Exp. (110), e52589, doi:10.3791/52589 (2016).
Copy