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Immunoistochimica e multiplo etichettatura con anticorpi delle specie ospite stessi allo Studio adulti ippocampale neurogenesi
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JoVE Journal 神経科学
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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Immunoistochimica e multiplo etichettatura con anticorpi delle specie ospite stessi allo Studio adulti ippocampale neurogenesi

26,336 Views

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09:24 min

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April 22, 2015

DOI:

10.3791/52551-v

DOI:
10.3791/52551-v

•

09:24 min
April 22, 2015

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1 Views
, , ,
1Hans Berger Department of Neurology,Jena University Hospital

概要

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This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

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Adult NeurogenesisImmunohistochemistryMultiple LabelingAntibodiesHippocampusNeural Stem CellsProgenitor SubtypesProliferation MarkersImmunofluorescenceConfocal MicroscopyCell QuantificationSequential Immunofluorescence

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Cite this Article

Ansorg, A., Bornkessel, K., Witte, O. W., Urbach, A. Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis. J. Vis. Exp. (98), e52551, doi:10.3791/52551 (2015).

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