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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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医学
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JoVE Journal 医学
High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

32,746 Views

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11:05 min

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January 21, 2015

DOI:

10.3791/52332-v

DOI:
10.3791/52332-v

•

11:05 min
January 21, 2015

•

31 Views
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1生物工学科,University of Illinois at Chicago, 2病理学科,University of Illinois at Chicago, 3Department of Biological Sciences,University of Illinois at Chicago, 4化学科,University of Illinois at Chicago, 5Department of Nephrology,University of Illinois at Chicago

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Fourier Transform Infrared (FT-IR) spectroscopic imaging is a fast and label-free approach to obtain biochemical data sets of cells and tissues. Here, we demonstrate how to obtain high-definition FT-IR images of tissue sections towards improving disease diagnosis.

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Sreedhar, H., Varma, V. K., Nguyen, P. L., Davidson, B., Akkina, S., Guzman, G., Setty, S., Kajdacsy-Balla, A., Walsh, M. J. High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology. J. Vis. Exp. (95), e52332, doi:10.3791/52332 (2015).

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