Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane

Omar Akil; Stephanie L. Rouse; Dylan K. Chan; Lawrence R. Lustig

doi:10.3791/52187

ジャーナル

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神経科学

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Método cirúrgico para Virally Mediated Gene entrega à orelha de rato Inner através da janela redonda Membrane

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JoVE Journal 神経科学

Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane

Método cirúrgico para Virally Mediated Gene entrega à orelha de rato Inner através da janela redonda Membrane

14,807 Views

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07:32 min

•

March 16, 2015

DOI:

10.3791/52187-v

DOI:

10.3791/52187-v

•

07:32 min

March 16, 2015

•

16 Views

Omar Akil¹, Stephanie L. Rouse¹, Dylan K. Chan¹, Lawrence R. Lustig¹

¹Department of Otolaryngology-Head and Neck Surgery,University of California, San Francisco

概要

The described post-auricular surgical approach allows rapid and direct delivery into the mouse cochlear scala tympani while minimizing blood loss and animal mortality. This method can be used for cochlear therapy using molecular, pharmacologic and viral delivery to postnatal mice through the round window membrane.

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Método cirúrgico para Virally Mediated Gene entrega à orelha de rato Inner através da janela redonda Membrane

概要

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