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Automatizzare esperimenti ChIP-seq per generare profili epigenetici su 10.000 cellule HeLa
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December 10, 2014
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概要
Automatically generated
Methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect of genomic research but they are laborious, costly, and time consuming. Here a commercially available robotic liquid handling system that automates chromatin immunoprecipitation for mapping in vivo protein-DNA interactions with limited amounts of cells is presented.
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Berguet, G., Hendrickx, J., Sabatel, C., Laczik, M., Squazzo, S., Mazon Pelaez, I., Saxena, R., Pendeville, H., Poncelet, D. Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells. J. Vis. Exp. (94), e52150, doi:10.3791/52150 (2014).
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