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Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells
DOI:

08:34 min

December 10, 2014

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Chapters

  • 00:05Title
  • 01:40Chromatin Immunoprecipitation and Library Preparation Assays
  • 05:48Results: Generation of Chromatin Epigenetic Profiles with as Few as 10,000 Cells
  • 07:38Conclusion

Summary

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Methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect of genomic research but they are laborious, costly, and time consuming. Here a commercially available robotic liquid handling system that automates chromatin immunoprecipitation for mapping in vivo protein-DNA interactions with limited amounts of cells is presented.

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