RNA interference (RNAi)-based gene knockdown techniques are at the core of Tribolium research. Here, we provide an overview of our larval RNAi technique in Tribolium castaneum. Larval RNAi is a simple, but powerful technique that provides quick access to loss-of-function phenotypes, allowing researchers to study gene functions in diverse contexts.
Linz, D. M., Clark-Hachtel, C. M., Borràs-Castells, F., Tomoyasu, Y. Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum. J. Vis. Exp. (92), e52059, doi:10.3791/52059 (2014).