ジャーナル
/
生物学
/
Methods to Assess Subcellular Compartments of Muscle in C. elegans
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Methods to Assess Subcellular Compartments of Muscle in C. elegans

Methods to Assess Subcellular Compartments of Muscle in C. elegans

DOI:
10.3791/52043-v

•

10:13 min

•

November 13, 2014

•

, , ,
1MRC/ARUK Centre for Musculoskeletal Ageing Research,University of Nottingham

章

  • 00:05標題
  • 01:50Synchronization of L1 Larval Animals Using Gravity Flotation
  • 03:08Movement Assays
  • 03:59Imaging of Mitochondrial Networks and Sarcomeres in Body Wall Muscle and Assessing Membrane Potential
  • 06:13Assessment of Mucle Protein Degradation
  • 07:57Results: The Effect of Muscle Disprution in C. elegans
  • 09:37Conclusion

概要

自動翻訳

自動翻訳

Skeletal muscle is essential for locomotion and is the bodies’ main protein store. Muscle health measurements within C. elegans are described. Prospective changes to muscle structure and function are assessed using localized GFP and cationic dyes.

タグ

MuscleC. ElegansMitochondriaSarcomeresGFPCationic DyesMuscle FunctionMuscle StructureMuscle HomeostasisMovement AssaysGenomic RegulationIn Vivo

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Generation of Stable Transgenic C. elegans Using Microinjection

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

C. elegans Chemotaxis Assay

Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae

Antibody Staining in C. Elegans Using "Freeze-Cracking"

An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans

Methods for Skin Wounding and Assays for Wound Responses in C. elegans

Read Article