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Intracerebroventricular Iniezione virale del cervello neonatale mouse per persistente e diffusa trasduzione neuronale
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神経科学
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JoVE Journal 神経科学
Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
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Intracerebroventricular Iniezione virale del cervello neonatale mouse per persistente e diffusa trasduzione neuronale

56,584 Views

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10:15 min

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September 15, 2014

DOI:

10.3791/51863-v

DOI:
10.3791/51863-v

•

10:15 min
September 15, 2014

•

82 Views
, , , ,
1神経科学科,Baylor College of Medicine, 2Department of Neuroscience, Center for Translational Research in Neurodegenerative Disease,University of Florida, 3Department of Neurology and Department of Neurosurgery,Baylor College of Medicine

概要

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Here we demonstrate a technique for widespread neuronal transduction by intraventricular injection of adeno-associated virus into the neonatal mouse brain. This method provides a rapid and easy way to attain lifelong expression of virally-delivered transgenes.

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IntracerebroventricularViral InjectionNeonatal Mouse BrainNeuronal TransductionAdeno-associated VirusAAVEpendymal LiningGene ExpressionFluorescent ProteinViral TiterStereotaxic Frame

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Kim, J., Grunke, S. D., Levites, Y., Golde, T. E., Jankowsky, J. L. Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction. J. Vis. Exp. (91), e51863, doi:10.3791/51863 (2014).

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