Protocols for efficient preparation of homogenous samples of spider mites, infestation of experimental plants, and assessment of plant damage, as required for studies of plant-pest interaction were developed.
Cazaux, M., Navarro, M., Bruinsma, K. A., Zhurov, V., Negrave, T., Van Leeuwen, T., Grbic, V., Grbic, M. Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies. J. Vis. Exp. (89), e51738, doi:10.3791/51738 (2014).