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Regia neuroni dopaminergici differenziazione da cellule staminali pluripotenti umane
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JoVE Journal 神経科学
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
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Regia neuroni dopaminergici differenziazione da cellule staminali pluripotenti umane

16,195 Views

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06:40 min

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September 15, 2014

DOI:

10.3791/51737-v

DOI:
10.3791/51737-v

•

06:40 min
September 15, 2014

•

13 Views
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1Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 2Department of Obstetrics and Gynecology,Stanford University School of Medicine

概要

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We, based on knowledge from developmental biology and published research, developed an optimized protocol to efficiently generate A9 midbrain dopaminergic neurons from both human embryonic stem cells and human induced pluripotent stem cells, which would be useful for disease modeling and cell replacement therapy for Parkinson’s disease.

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Dopaminergic NeuronsPluripotent Stem CellsParkinson’s DiseaseCell Replacement TherapyFloor PlateVentral MidlineNeural DevelopmentDifferentiationHuman Embryonic Stem CellsHuman Induced Pluripotent Stem CellsIn Vitro Disease ModelingDrug ScreeningCell Transplantation

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Cite this Article

Zhang, P., Xia, N., Reijo Pera, R. A. Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells. J. Vis. Exp. (91), e51737, doi:10.3791/51737 (2014).

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