A Protocol to Infect <em>Caenorhabditis elegans</em> with <em>Salmonella typhimurium</em>

Jiuli Zhang; Kailiang Jia

doi:10.3791/51703

ジャーナル

/

免疫学と感染

/

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

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免疫学と感染

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JoVE Journal 免疫学と感染

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

DOI:

10.3791/51703-v

•

06:50 min

•

June 26, 2014

•

Jiuli Zhang, Kailiang Jia

¹Department of Biological Sciences,Florida Atlantic University

章

00:05標題
00:53Preparations
02:01Feeding RNAi to Nematodes
03:34Treating C. elegans with Salmonella and Scoring Survival
05:41C. elegans Survival from Salmonella Infection and bec-1
06:25Conclusion

概要

自動翻訳

C. elegans has emerged as a new genetic model to study host-pathogen interactions. Here we describe a protocol to infect C. elegans with Salmonella typhimurium coupled with the double-strand RNAi interference technique to examine the role of host genes in defense against Salmonella infection.

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